Biostasis Roadmap

A research and technology development roadmap for preservation and recovery of brains (and bodies).
Click here to read the Biostasis Roadmap Whitepaper.
Partners
✨
General
↳
Maintaining circulatory system health
1️
?
↳
Anticoagulation protocols
2
$$
↳
Protocol changes based on medical conditions
3
$$
↳
Health monitoring
4
$$
♥️ PRE CARDIAC ARREST
🚑 POST  CARDIAC ARREST STABILIZATION
✨
General
a.
Monitoring
↳
Cardiac arrest notification
1️
$$
b.
Measure of stabilization procedure success
↳
S-MIX value
1️
$$
🧊
Cryopreservation
a.
Acute stabilization protocol
↳
Stabilization medications
1️
$$
↳
Stabilization cooling
2
$$
↳
Field Cardiopulmonary Bypass (CPB)
3
$$
↳
Stabilization Blood Substitution / Transport
4
$$
b.
Abbreviated stabilization (long ischemic time)
↳
Criteria for Abbreviated Protocol vs Acute Protocol
1
$
↳
Medications
2
$
↳
Medication circulation
3
$
↳
Oxygenation after long ischemic time
4
$$
🧪
Chemical Fixation
a.
Stabilization for chemical fixation-based preservation
↳
Medications/Circulation/Cooling/Transport
1️
$$
💧 PRESERVATION COMPOUNDS
✨
General
a.
Interventions and additives to improve perfusion quality
↳
High osmolarity chemicals
1️
$
↳
Blood-brain barrier modifiers (chemical)
2
$
↳
Blood-brain barrier modifiers (ultrasound)
3
$
↳
Blood clot dispersal agents
4
$
🧊
Cryopreservation
a.
Chemical cryoprotectant agents
↳
New cryoprotectants and new mixtures
1️
$$
↳
Lower critical cooling rate
2
$$
↳
Lower critical warming rate
3
$$
↳
Lower toxicity
4
$$
↳
Better perfusability
5
$$
b.
Other additives
↳
Cryoprotective proteins or biologically-insipred agents
1
$$
↳
Oxygenation of perfusate
2
$
🧪
Chemical Fixation
a.
Aldehydes
↳
Aldehyde chemical used
1️
$
↳
Optimize biomolecular preservation
2
$$
↳
Chemicals to accelereate immersion fixation
3
$
b.
Non-aldehyde crosslinkers
↳
Cleavable with contemporary technology
1
$$
c.
Long-term fluid preservatives
↳
Better maintenance of morphology and biomolecules
1
$$
📝 PROCEDURES
✨
General
a.
Surgical & cannulation technique
↳
Transcardiac
1️
$$
↳
Aortic route
2
$$
↳
In situ cardiod roue
3
$$
↳
Cephalon isolated routes
4
$$
↳
Ex situ cerebral vessel cannulation
5
$$
↳
Optimal perfusion pressure curve
6
$
↳
Protocol changes based on patient conditions
7
$$
b.
Preventing air microemboli in brain vessels
↳
Quality of perfusion in realistic cases
1
$
c.
Burr hole creation
↳
Maximize speed while minimizing risk
1
$
d.
Brain extraction
↳
Maximize speed while minimizing trauma
1
$
🧊
Cryopreservation
a.
Rapid cooling techniques
↳
Procedure for fast cooling of large volume
1️
$
b.
Perfusion of cryoprotectants
↳
Improve perfusion procedures
1
$
c.
Persufflation
↳
Persuffation
1
$$
c.
Fracturing
↳
Cryogenic cooling protocols to reduce thermal stress
1
$$
d.
Unprotected cryopreservation
↳
Quantify extent of damage due to ice formation
1
$$
🧪
Chemical Fixation
a.
Immersion fixation
↳
Benchmark existing immersion methods
1️
$
↳
Procedural acceleration of immersion fixation
2
$
b.
Perfusion fixation
↳
Necessity of washout solutions
1
$$
c.
Post-fixation cryoprotectant delivery
↳
Perfusion-based delivery
1
$
↳
Immersion-based delivery
2
$
📏 QUALITY METRICS
✨
General
a.
Microscopic appearance of cells
↳
Light microscopy-level detail metrics
1
$
↳
Ultrastructural-level detail metrics
2
$$
↳
Biomolecular annotation metrics
3
$$
↳
Inferability of structural appearance
4
$$
🧊
Cryopreservation
a.
Intra preservation quality standards
↳
Refractive Index at end of perfusion
1️
$
↳
Dehydration
2
$
b.
Post preservation quality standards
↳
CT-based quality metrics
1
$
c.
Post-devitrification measurements
↳
Post devitrification structural preservation
1
$$
↳
Post devitrification cellular viability
2
$$
↳
Post devitrification neural activity
3
$$
⏳ LONGTERM PRESERVATION
🧊
Cryopreservation
a.
Temperature of storage
↳
Temperature of storage
1️
$
b.
Long-term quality
↳
Establish long term quality metrics
1
$
🧪
Chemical Fixation
a.
Post-fixation cryoprotection and sub-zero storage
↳
Post-fixation cryoprotection and sub-zero storage
1️
$
b.
Long-term fluid preservation
↳
Optimize fluid preservation
1
$$
✅ RESTORATION
✨
General
a.
Repair of general damage
↳
Repair of general damage
1️
$$$$
🧊
Cryopreservation
a.
Rewarming
↳
Dielectric warming
1️
$$$
↳
Nano-rewarming
2
$$$
↳
Ultrasound rewarming
3
$$$
↳
Chemical rewarming
Chemical rewarming
4
$$$
↳
Novel rewarming technology
5
$$$
🧪
Chemical Fixation
a.
Crosslinking reversal
↳
Chemical technology
1️
$$$$
a.
Whole brain emulation
↳
Mapping brains at the connectome level
1️
$$$$
↳
Whole brain emulation of small organisms
2
$$$$